Cat# YRB100
Total RNA Extraction Kit (Blood/Bacteria/Cultured Cells)
Reliable RNA purification
Total RNA Extraction Kit (Blood/Bacteria/Cultured Cells)
Reliable RNA purification
The Total RNA Extraction Kit (Blood/Bacterial/Cultured Cells) is specially designed for purification of total RNA from bacterial, cultured cells and fresh human whole blood. The method uses detergents and a chaotropic salt to lyse the cells and inactivate RNase. The lysate is applied to provided Lysate Filter columns (Mini System) to remove cell debris and ensure complete cell lysis. RNA in chaotropic salt solutions binds to the glass fiber matrix of the columns. Following washing off of contaminants, the purified RNA is eluted by RNase-free water. ssRNA and dsRNA of > 200 bps to 1000′s of bps in length are efficiently purified.
Storage and Stability: Store at Room Temperature
Packing: 100 mini preps / Kit
Protocol: "RNA Extraction Kit(YRB YRBM YRT YRP YRPM) protocol V2013-01"
Sample (Protocols Included): Whole Blood/Buffy Coat, cultured animal cell, gram -/+ bacteria.
Sample size: >300μl of human whole blood, 106 cultured mammalian cells, 108 bacterial cells.
Yield: up to 30μg.
For complete genomic DNA removal , DNase I (RNase-free) must be added.
|
RB Column Set |
100 columns |
|
Filter Column Set |
100 tubes |
|
RBC Lysis Buffer |
240 ml |
|
RB Buffer |
60 ml |
|
RT Buffer |
30 ml |
|
R-W1 Buffer |
50 ml |
|
R-Wash Buffer |
25 ml |
|
RNase-Free Water |
10 ml |