Cat# YPD96B-2
96-Well Plasmid Kit
Ready to Use Total Solution (Centrifuge/Centrifuge and Vacuum combined Protocol)

The 96-well Plasmid Kit is designed for rapid high throughput isolation of plasmid or cosmid DNA from 1- 2 ml of bacterial cultures. All components and reagents are provided for a complete solution. In the process, the modified alkaline lysis method and RNase A treatment are used to obtain cleared cell lysate with minimal genomic DNA and RNA contaminants. In the presence of a chaotropic salt, the plasmid DNA in the lysate binds to glass fiber matrix in the Plasmid Binding Plate. The contaminants are washed off with an ethanol-based wash buffer and finally, the purified plasmid DNA is eluted by low salt elution buffer or water. The protocol does not require DNA phenol extraction or alcohol precipitation. Typical yields are 5-10 μg for high-copy number plasmid and 0.5-5μg for low-copy number plasmids. The entire procedure can be completed within 60 minutes and the purified plasmid DNA is ready for restiction digestion, ligation, PCR, and sequencing reaction.

Storage and Stability: Store at Room Temperature

Packing: 2x96 preps / Kit
Protocol: "Plasmid mini Kit(YPD YPI YPM YPD96B) protocol V3.0"

Sample Source: Plasmid DNA from Bacteria

Sample Size: 1-2ml of bacterial cultures

Typical Plasmid Yield : 
Low Copy Number: 0.5-5μg 
High Copy Number: 5-10μg
Preparation time: 60min