Category: Molecular Biology
Manufacturer: Bio-Lab

Cat# 9597580SM2700
250 µl x 2
5 µl per well for 100 gel applications

The SuperMarker2700 3-color Pre-Stained Protein Marker is a ready to use three-color protein standard with 13 pre-stained proteins covering a wide range of molecular weights from 5 to 245 kDa in Tris-Glycine buffer (3.5 to 235 kDa in Bis-Tris (MOPS) buffer and Bis-Tris (MES) buffer). Proteins are covalently coupled with different chromophores for easy identification of bands, with two reference proteins carrying enhanced intensity corresponding to a green at 25 kDa and red at 75 kDa, respectively, as separated on SDS-PAGE (Tris-Glycine buffer).

The SuperMarker2700 3-color Pre-Stained Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.
The marker is supplied in gel loading buffer and is ready to use. Do NOT heat, dilute and add reducing agent before loading.


Storage and Stability: 4°C ≥3 months, 20°C  ≥24 months
Packing: 250 µl x 2

Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25℃), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).

Loading Volume:
3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively
1.5~2.5 μl per well for general Western transferring
Apply more for thicker (> 1.5 mm) or larger gel

10 mM Tris-HCl (pH 8.0), 10 mM Na-EDTA, 0.025% Orange G, 0.005% Xylene cyanol FF and 10% Glycerol.

Quality Control:
12 defined bands are observed during electrophoresis in agarose gel.
No nuclease activity is detected by a direct nuclease activity assay.