Cat# 9597580SL3100
500 µl
5 µl per well for 100 gel applications
Description:
The SuperLadder3100 1 KB (0.25-10 kb) DNA Ladder is a ready-to-use DNA ladder, which is pre- mixed with loading dye for direct gel loading. The DNA ladder is composed of 13 individual DNA fragments: 10k, 8k, 6k, 5k, 4k, 3k, 2.5k, 2k, 1.5k, 1k, 750, 500, and 250 bp derived from a mixture of PCR products and specifically digested plasmid DNA. This product contains two enhanced bands (3 kb and 1 kb) for easy reference. In addition, two tracking dyes, Xylene cyanol FF and Bromophenol blue which mimic the migration of 4,000 bp and 500 bp dsDNA during electrophoresis are also added for real time monitoring.
The SuperLadder3100 1 KB (0.25-10 kb) DNA Ladder is a ready-to-use DNA ladder, which is pre- mixed with loading dye for direct gel loading. The DNA ladder is composed of 13 individual DNA fragments: 10k, 8k, 6k, 5k, 4k, 3k, 2.5k, 2k, 1.5k, 1k, 750, 500, and 250 bp derived from a mixture of PCR products and specifically digested plasmid DNA. This product contains two enhanced bands (3 kb and 1 kb) for easy reference. In addition, two tracking dyes, Xylene cyanol FF and Bromophenol blue which mimic the migration of 4,000 bp and 500 bp dsDNA during electrophoresis are also added for real time monitoring.
Storage and Stability: Room temperature ≥ 6 months, 4°C ≥ 3 months, -20°C ≥ 24 months
Packing: 500 µl
Protocol: SuperLadder3100.
Contents:
Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25℃), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).
Buffering:
10 mM Tris-HCl (pH 8.0), 10 mM Na-EDTA, 0.005% Bromophenol blue, 0.005% Xylene cyanol FF and 10% Glycerol.
Quality Control:
12 defined bands are observed during electrophoresis in agarose gel.
No nuclease activity is detected by a direct nuclease activity assay
.