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WESTAR Supernova

In the field of Western Blotting and ELISA, Cyanagen's patented discovery of the 'secondary enhancer' (enzyme activator) is the basis of its WESTAR and ELISTAR lines.

Protocol

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Introduction

In the field of Western Blotting and ELISA, Cyanagen’s patented discovery of the 'secondary enhancer' (enzyme activator) is the basis of its WESTAR and ELISTAR lines. Cyanagen holds several international patents that cover both the production process of the most important starting material, a phenothiazine derivative, and the final products themselves. Cyanagen’s technology allows for both the optimization of the product performance, and the fine and continuous adjustment of the intensity and duration of the chemiluminescent signal.

Advantages

  • Safe to use
  • Easy to prepare
  • Immediate light generation
  • Wide linear dynamic range

 

Features

 

Features:

Westar Supernova substrate is readily prepared by mixing its two components, a luminol/enhancer solution and a peroxide solution in a one-to-one ratio.

Components:

Luminol/enhancer solution (A)
Peroxide solution (B)

Antibody Dilution Range:

Primary: 1:5,000-1:100,000
Secondary: 1:100,000-1:500,000
(from 1 mg/mL stock solution)

Available as:

2x10 mL kit (200 cm2 of membrane)
2x50 mL kit (1,000 cm2 of membrane)
2x100 mL kit (2,000 cm2 of membrane)

Sensitivity:

Low femtogram (10-15)

Signal duration:

8 hours

Storage:

Stable for 1 year at 4 °C. Product is shipped at ambient temperature.

 

Protocols

 

Protocol

MSDS solution A 

MSDS solution B

 

Products


Products

Order-No

Substrate

Signal intensity

Protein quantity

Primary Ab dilution range*

Secondary Ab dilution range*

XLS063

WESTAR ECL-SUN

Standard

High abundance

From 1/100 to 1/5,000

From 1/1,000 to 1/15,000

XLS10

WESTAR Nova 2011

Medium

High abundance

From 1/500 to 1/5,000

From 1/20,000 to 1/100,000

XLS100

WESTAR EtaC

High

Medium abundance

From 1/1,000 to 1/15,000

From 1/25,000 to 1/150,000

XLS4

WESTAR EtaC Ultra

Very High

Low abundance

From 1/5,000 to 1/50,000

From 1/50,000 to 1/250,000

XLS3

WESTAR Supernova

Extreme

Very-low abundance

From 1/5,000 to 1/100,000

From 1/100,000 to 1/500,000

XLS025

WESTAR Starter Kit

WESTAR Starter kit is composed by all WESTAR as sample quantity.
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