QPRO – BCA kit Standard
- Category: Molecular Biology

Cyanagen's Protein Quantitation line includes Qpro and mQpro. They are Bicinchoninic Acid (BCA) - based protein assay (BCA assay) for highly sensitive colorimetric analysis, compatible with detergent solubilized protein solutions.
Introduction
Cyanagen’s Protein Quantitation line includes Qpro and mQpro.
They are Bicinchoninic Acid (BCA) - based protein assay (BCA assay) for highly sensitive colorimetric analysis, compatible with detergent solubilized protein solutions.
The BCA method is fast and easy, providing enhanced flexibility with much greater tolerance to interferences by non-ionic detergents and buffer salts.
Advantages
- Broad linear working ranges with excellent sensitivity
- Can be used with tubes and microplates
- Less protein-to-protein variation than dye-binding methods
- Unaffected by most ionic and nonionic detergents such as Triton-100 and SDS (1%)
- Much easier and faster than the classical Lowry method
- Solution A: 1 L of 1% BCA/tartrate in alkaline carbonate buffer
- Solution B: 25 mL of 4% CuSO4 5H2O in water
- Solution A: 1 L of 1% BCA/tartrate in alkaline carbonate buffer
- Solution A: 240 mL of tartrate in alkaline carbonate buffer
- Solution B: 240 mL of 4% BCA in water
- Solution C: 12 mL of 4% CuSO4 5H2O in water
Features
Features: |
BCA protein assay for 20-2000 μg/mL rotein.Proteins reduce alkaline Cu(II) to Cu(I). Bicinchoninic acid forms an intense purple complex with cuprous ion with an absorbance maximum at 562 nm. The absorbance is directly proportional to protein concentration. A detailed protocol of use is included. |
Sufficient for: |
500 test tube or 5,000 microplate assays |
Components: |
Reagent A (BCA/tartrate in alkaline carbonate buffer) 1 L |
Storage: |
Room Temperature |
Protocols
Products
Products
Order-No |
Product Description |
Quantity |
QPRO – BCA kit Standard |
||
µQPRO – BCA kit Micro |
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