Introduction
Cyanagen’s Protein Quantitation line includes Qpro and mQpro.
They are Bicinchoninic Acid (BCA) - based protein assay (BCA assay) for highly sensitive colorimetric analysis, compatible with detergent solubilized protein solutions.
The BCA method is fast and easy, providing enhanced flexibility with much greater tolerance to interferences by non-ionic detergents and buffer salts.
Advantages
- Broad linear working ranges with excellent sensitivity
- Can be used with tubes and microplates
- Less protein-to-protein variation than dye-binding methods
- Unaffected by most ionic and nonionic detergents such as Triton-100 and SDS (1%)
- Much easier and faster than the classical Lowry method
- Solution A: 1 L of 1% BCA/tartrate in alkaline carbonate buffer
- Solution B: 25 mL of 4% CuSO4 5H2O in water
- Solution A: 1 L of 1% BCA/tartrate in alkaline carbonate buffer
- Solution A: 240 mL of tartrate in alkaline carbonate buffer
- Solution B: 240 mL of 4% BCA in water
- Solution C: 12 mL of 4% CuSO4 5H2O in water