Total RNA Extraction Kit [Maxi] (Blood/Bacteria/Cultured Cells)-25

Category: Nucleic Acid Purification
Manufacturer: RBC Bioscience
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Cat# YRBM25
Total RNA Extraction Kit [Maxi] (Blood/Bacteria/Cultured Cells)
Reliable RNA purification

rbc

The Total RNA Extraction Kit (Blood/Bacterial/Cultured Cells) is specially designed for purification of total RNA from bacterial, cultured cells and fresh human whole blood. The method uses detergents and a chaotropic salt to lyse the cells and inactivate RNase. The lysate is applied to provided Lysate Filter columns (Mini System) to remove cell debris and ensure complete cell lysis. RNA in chaotropic salt solutions binds to the glass fiber matrix of the columns. Following washing off of contaminants, the purified RNA is eluted by RNase-free water. ssRNA and dsRNA of > 200 bps to 1000′s of bps in length are efficiently purified.

Storage and Stability: Store at Room Temperature
Packing: 25 maxi preps / Kit
Protocol: "RNA Extraction Kit(YRB YRBM YRT YRP YRPM) protocol V2013-01"

Sample (Protocols Included): Sample 100-200 mg animal tissue, 5 ml blood sample
Yield: up to 500 μg.
Operation time: < 60 min
Elution volume: 500 μl
For complete genomic DNA removal DNase I (RNase-free) must be added.

YRBM252

RB maxi Column Set

25 columns

Filter Maxi Column Set

25 tubes

RBC Lysis Buffer

1000 ml

RB Buffer

150 ml

RT Buffer

60 ml

R-W1 Buffer

130 ml

R-Wash Buffer

40 ml

RNase-Free Water

30 ml