96-Well Plasmid Kit (Centrifuge/centrifuge & Vacuum)-10

Category: Nucleic Acid Purification
Manufacturer: RBC Bioscience
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Cat# YPD96B-10
96-Well Plasmid Kit (Centrifuge/centrifuge & Vacuum)

YPD100
The 96-well Plasmid Kit is designed for rapid high throughput isolation of plasmid or cosmid DNA from 1- 2 ml of bacterial cultures. All components and reagents are provided for a complete solution. In the process, the modified alkaline lysis method and RNase A treatment are used to obtain cleared cell lysate with minimal genomic DNA and RNA contaminants. In the presence of a chaotropic salt, the plasmid DNA in the lysate binds to glass fiber matrix in the Plasmid Binding Plate. The contaminants are washed off with an ethanol-based wash buffer and finally, the purified plasmid DNA is eluted by low salt elution buffer or water. The protocol does not require DNA phenol extraction or alcohol precipitation. Typical yields are 5-10 μg for high-copy number plasmid and 0.5-5μg for low-copy number plasmids. The entire procedure can be completed within 60 minutes and the purified plasmid DNA is ready for restiction digestion, ligation, PCR, and sequencing reaction.

Storage And Stability: Store at Room Temperature

Packing: 19x96 preps / Kit

Protocol: "Plasmid mini Kit (YPD YPI YPM YPD96B) protocol V3.0"

Sample Source: Plasmid DNA from Bacteria

Sample Size: 1-2ml of bacterial cultures
Typical Plasmid Yield
Low Copy Number: 0.5-5μg 
High Copy Number: 5-10μg
Preparation time: 60min 

 

96-well 2 ml Sample Plate

20 pcs

96-well PCR Plate

25 pcs

0.35ml Plasmid Binding Plate

10 pcs

PDA1 Buffer

110 ml

PDA2 Buffer

110 ml

PDA3 Buffer

180 ml

W1 Buffer

400 ml

Wash Buffer

125 ml

Elution Buffer

100 ml

RNase A

220 µl