Western Blotting

QPRO – BCA Acid Solution

Category: Molecular Biology
Manufacturer: Cyanagen
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Introduction 

Cyanagen’s Protein Quantitation line includes Qpro and mQpro.

They are Bicinchoninic Acid (BCA) - based protein assay (BCA assay) for highly sensitive colorimetric analysis, compatible with detergent solubilized protein solutions.

The BCA method is fast and easy, providing enhanced flexibility with much greater tolerance to interferences by non-ionic detergents and buffer salts.

Advantages 

  • Broad linear working ranges with excellent sensitivity
  • Can be used with tubes and microplates
  • Less protein-to-protein variation than dye-binding methods
  • Unaffected by most ionic and nonionic detergents such as Triton-100 and SDS (1%)
  • Much easier and faster than the classical Lowry method
  • Solution A: 1 L of 1% BCA/tartrate in alkaline carbonate buffer
  • Solution B: 25 mL of 4% CuSO4 5H2O in water
  • Solution A: 1 L of 1% BCA/tartrate in alkaline carbonate buffer
  • Solution A: 240 mL of tartrate in alkaline carbonate buffer
  • Solution B: 240 mL of 4% BCA in water
  • Solution C: 12 mL of 4% CuSO4 5H2O in water

Features:

Proteins reduce alkaline Cu(II) to Cu(I). Bicinchoninic acid forms an intense purple complex with cuprous ion with an absorbance maximum at 562 nm. The absorbance is directly proportional to protein concentration.

Sufficient for:

500 test tube or 5,000 microplate assays

Components:

Reagent A (BCA/tartrate in alkaline carbonate buffer) 1 L

Storage:

Room Temperature

Products

Order-No

Product Description

Quantity

PRTD1,1000

QPRO – BCA kit Standard
20÷2000 mg/ml protein

 

PRTD1A,1000

 

PRTD2,0500

µQPRO – BCA kit Micro
0.5÷20 mg/ml protein