ExcelBand 3-color Regular Range Protein Marker

Category: Molecular Biology
Manufacturer: Bio-Lab
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Cat# 9597580SM2500 ExcelBand 3-color Regular Range Protein Marker

sm2500wb

Description

The SuperMarker2500 ExcelBand Enhanced 3-color Regular Range Protein Marker is a ready-to-use three-color protein standard with 10 pre-stained proteins covering a wide range of molecular weights from 10 to 180 kDa in Tris-Glycine Buffer (9 to 170 kDa in Bis-Tris (MOPS) buffer and 10 to 180 kDa Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-Glycine buffer). SuperMrker2500 ExcelBand Enhanced 3-color Regular Range Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.

Contents

Approximately 0.2~0.6 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5), 2% SDS, 0.2 mM DTT, 3.6 M urea, and 15% (v/v) glycerol).

Quality Control

Under suggested conditions, SM2500 ExcelBand Enhanced 3-color Regular Range Protein Marker resolves 10 major bands in 15% SDS-PAGE (Tris-Glycine buffer) and after Western blotting to nitrocellulose membrane.

Storage
4°C ≥ 3 months
-20°C ≥ 24 months

Protocol

 click here.