Genomic DNA Extraction Kit (Blood/Bacteria/Cultured Cells)-300

Category: Nucleic Acid Purification
Manufacturer: RBC Bioscience
Cat# YGB300
Genomic DNA Extraction Kit (Blood/Bacteria/Cultured Cells)
Mini/Maxi Format
Genomic DNA Extraction Kits (Blood/Bacteria/Cultured Cells) provides a fast and economical method for purification of total DNA (including genomic, mitochondrial and viral DNA) from whole blood, plasma, serum, buffy coat, other body fluids, lymphocytes and cultured cells. This kit is also ideal for bacteria and cultured cells. The blood protocol utilises RBC lysis buffer, GB buffer and a rapid heating step to release DNA into solution. DNA in the chaotropic salt solution binds to the glass fiber matrix of column. After washing off the contaminants, the purified DNA is eluted by low salt elution buffer or water. There is no requirement for phenol/chloroform extraction or alcohol precipitation. Purified DNA of approximately 20-30 kb is suitable for PCR or other enzymatic reactions.

Storage and Stability: Store at Room Temperature
Packing: 300 mini preps / Kit
Protocol: "Genomics Extraction Kit(YGB YGBM YGT YGP YGPM) protocol V2013-1"

: 300µl Whole Blood (up to 1ml), 107 Cultured Cells, 109 Bacteria (Gram +/-), 107 Yeast
Average Yields: 200µl Whole Blood-6µg, 200µl Buffy-50µg, 5x106 Lymph./Cult. Cells-50µg





GD Column

300 column

2 ml Collection Tube

300 tubes

W1 Buffer

130 ml

Wash Buffer

40 ml

Elution Buffer

60 ml

RBC Lysis Buffer

500 ml

GB Buffer

100 ml

GT Buffer

100 ml

Proteinase K

66 mg